This study provides a checklist of the Acalyphoideae (Euphorbiaceae) present in Equatorial Guinea, comprised of 18 genera and 49 taxa. Identification keys have been added for genera and species of the subfamily. The best represented genus is Macaranga with ten species. Bibliographical references for Acalyphoideae (Euphorbiaceae) from Equatorial Guinea have been gathered and checked. Eight taxa are recorded for the first time from the country. One species is included based on literature records, because its distribution ranges suggest it may occur in Equatorial Guinea, and two introduced species could be naturalized.